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Bridge RNAs direct programmable recombination of focus on and donor DNA A bispecific non-coding RNA expressed from the IS110 spouse and children of cell genetic factors forms the basis of the programmable genome-editing method that allows the insertion, excision or inversion of particular focus on DNA sequences.Scientists consistently use recombin
empty vector strain no matter media or anthrose status in the reporter strain. In BHI, when the atxAThe NEBuilder HiFi DNA assembly kit was utilized to assemble the plasmid backbone and anthrose operon by Gibson assembly in accordance with the producer’s recommendations. Suitable insertion was confirmed by double-digest with the insert with EcoRI
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